Patients who use catheters for > 7 days have a 25% higher risk of developing a UTI, 5% of which are potentially dangerous. This increases the likelihood of bacterial adhesion and interference with the host defence system, resulting in an overactive bladder and incomplete urination, enabling the proliferation of residual microorganisms. A catheter may damage the protective urinary epithelial mucosa during insertion into the urethra, exposing the bacterial adhesion binding site. Most nosocomial urethral infections are associated with the use of catheters. pneumoniae causes 6–17% of urethral infections in hospitals. Approximately 20–50% of urethral infections are reported to be nosocomial infections acquired in intensive care units K. In conclusion, PM78, PM229 and RY2 showed the strongest inhibitory activity against urinary tract pathogens and could be suitable for use in the treatment of urinary tract infections.Įvery year, an estimated one billion women are affected by genitourinary infections including urinary tract infections (UTIs) and bacterial vaginosis caused by Klebsiella pneumoniae and Gardnerella vaginalis. PM68, PM78, PM201, PM206 and PM229 inhibited the invasion of SV-HUC-1 cells by G. In a competition assay, PM2 and RY2 inhibited urinary tract pathogens. The survival rate of urinary tract pathogens was 0–10.65% and pH of the culture medium decreased after co-culture with LAB strains for 4 h. After enzyme treatments, the supernatants showed first- or second-order inhibitory effects on K.

However, antibacterial activity disappeared entirely at pH 7.0. The results showed that the supernatants had good heat stability.

Anti-adhesion and anti-invasion activities against urinary tract pathogens were evaluated by SV-HUC-1 uroepithelium cell culture. Anti-growth activity against urinary tract pathogens was evaluated by co-culture inhibition assay using seven LAB strains. We studied the effect of heat, pH and enzyme treatment on the inhibitory activity of LAB strain supernatants.

We screened 370 LAB strains using spent culture supernatants by inhibition zone assay to assess their antimicrobial effects. The purpose of the present study was to identify strains of lactic acid bacteria (LAB) which could be used as alternatives to antibiotics for the treatment of urinary tract infections because of their ability to inhibit urinary tract pathogens ( Klebsiella pneumoniae BCRC 10694 and Gardnerella vaginalis BCRC 17040). Urinary tract infections are the most common infectious diseases in babies and the elderly and are often acquired as nosocomial infections.

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